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ZC BIOSCIENCERAA ISOTHERMAL DETECTION
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Gloved hand holding an 8-tube strip of lyophilised RAA reagent pellets
RAA isothermal nucleic acid detection

Molecular results in 5–15 minutes. No thermal cycler.

Recombinase-Aided Amplification (RAA) runs at a constant 37–42 °C. Lyophilised reagents, a portable fluorescence analyzer and a two-step workflow bring nucleic acid testing to the bench and to the field.

Request a Quote Explore RAA
5–15 minTime to fluorescence result
37–42 °CIsothermal, no cycling
LyophilisedReady-to-use dry pellets
3.8 kgPortable 8-well analyzer
RAA Core Amplification Kits6 enzyme reagent kits (RUO) Genchek Isothermal Fluorescence Analyzer8 wells · FAM & ROX Rapid Nucleic Acid Extraction Kits4 kits · 5–25 min prep (RUO) Food Safety Molecular Detection Series17 catalogue kits · 3 groups (RUO) Fully Automated Isothermal NAT SystemSample-in, result-out · ~30 min
RAA technology

Recombinase-Aided Amplification — a fast isothermal alternative to PCR

RAA is the isothermal amplification platform behind every ZC Bioscience reagent. All kits are developed and manufactured in-house.

Stylised blue DNA double helix on white

How RAA works

RAA is a rapid isothermal nucleic acid amplification technology. A recombinase obtained from bacteria or fungi binds tightly to the primer DNA at ambient temperature, forming a recombinase–primer complex. When the primer locates its fully complementary sequence on the template, single-stranded DNA-binding protein helps open the double strand, and DNA polymerase synthesises a new complementary strand. The product accumulates exponentially.

With a labelled fluorescent probe the reaction is read in real time; a fluorescence result is typically available within 5–15 minutes. All reaction components are pre-mixed and lyophilised in the tube — add Buffer A, Buffer B and the extracted sample nucleic acid, then place the strip in the analyzer. RAA can in principle be multiplexed: two or more primer pairs in one tube amplify several targets simultaneously.

See RAA reagent kits
  • Simple operationTwo-step workflow; molecular-level testing that does not require specialist staff.
  • FastResults in 5–15 minutes — truly rapid testing.
  • AccurateHigh sensitivity and specificity; precise recognition of the target sequence.
  • PortableCompact instruments suited to harsh field conditions and on-site inspection.
  • IsothermalRuns at 30–42 °C (optimum 37–39 °C); no thermal cycler or other expensive equipment required.
  • LyophilisedFreeze-dried pellets, ready to use, easy to ship, free from freeze–thaw loss.

Platform comparison

RAA compared with conventional PCR and LAMP on enzymes, temperature, time and readout.

TechnologyPCRLAMPRAA
EnzymesTaq DNA polymeraseStrand-displacing DNA polymeraseRecombinase, SSB, strand-displacing DNA polymerase
Reaction temperatureHigh, thermal cycling60–65 °C37–39 °C, constant
Reaction time1.5 h40 min15 min
Primer pairs121
Reagent formatLiquidLiquidLyophilised pellet
ReadoutGel; real-time fluorescenceVisual turbidityGel; real-time fluorescence; lateral-flow strip
LimitationSlowAerosol, false positives—
Products

Reagents, extraction and instruments for one RAA workflow

29 catalogue items across five product lines. All reagents are for research use only (RUO). Quotations are prepared per product and quantity.

8-tube strip of lyophilised RAA amplification reagent

RAA Core Amplification Kits (RUO)

Lyophilised enzyme reagents for DNA or RNA targets with gel, real-time fluorescence or lateral-flow readout. 48 tests/kit, 50 μL reaction, over 10⁷-fold amplification. Primers 30–35 bp, probes 46–52 bp. Store at −20 °C; 12 months.

  • RAA Basic Nucleic Acid Amplification Kit (DNA, gel) (RUO)S001ZC
  • RAA Fluorescent Nucleic Acid Amplification Kit (DNA, real-time) (RUO)S002ZC
  • RT-RAA Basic Nucleic Acid Amplification Kit (RNA, gel) (RUO)S003ZC
  • RT-RAA Fluorescent Nucleic Acid Amplification Kit (RNA, real-time) (RUO)S004ZC
  • RAA-nfo Nucleic Acid Amplification Kit (lateral-flow, DNA) (RUO)S005ZC
  • RT-RAA-nfo Nucleic Acid Amplification Kit (lateral-flow, RNA) (RUO)S006ZC
Compatible with Genchek-1/2 and standard real-time instrumentsRequest a Quote
Genchek isothermal fluorescence analyzer, white benchtop unit with touchscreen and 8-well block

Genchek Isothermal Fluorescence Analyzer

An integrated, portable rapid-test analyzer for on-site and mobile testing. Built-in reagent mixing matched to RAA reagents; dual fluorescence channels (FAM & ROX) — up to 16 targets per run; automatic analysis and report generation with multiple export routes.

Throughput8 wells
ChannelsFAM / ROX
Temperature rangeAmbient to 65 °C
Accuracy±0.5 °C
Net weight3.8 kg
Dimensions255 × 240 × 123 mm
Power120 W, AC 100–240 V
Operating5–35 °C, 20–80 % RH
25+ assays: species, pathogens, GMO elementsRequest a Quote
Gloved hand holding an 8-tube reagent strip

Rapid Nucleic Acid Extraction Kits (RUO)

Purpose-built sample prep that yields RAA-ready nucleic acid from animal tissue, blood, meat products, plant tissue, seeds, bacteria, swabs and feed. No hazardous solvents, low cross-contamination risk. Store at room temperature; 12 months.

  • Rapid DNA Extraction Reagent, heat-lysis, 5 min · 50 preps (RUO)ND01ZC
  • Universal Rapid DNA Extraction Kit, spin column, 15 min · 50 preps (RUO)ND02ZC
  • Universal Rapid RNA Extraction Kit, spin column, 10 min · 50 preps (RUO)ND03ZC
  • Magnetic-Bead DNA Extraction Pre-filled Plate, 25 min · 96 preps (RUO)ND06ZC
Lyse – bind – wash – eluteRequest a Quote
Magnifying glass over a blue DNA helix

Food Safety Molecular Detection Series (RUO)

RAA fluorescent kits that recognise and amplify a target-specific gene fragment at 30–42 °C (optimum 37–39 °C). Result read directly from the amplification curve — no electrophoresis. 24 tests/kit; store at −20 °C; 6 months. Compatible with Genchek-I/II and standard real-time instruments (ABI, MJ, Roche, Bioer series).

Animal-species (meat authentication) kits (RUO)

10 kits · A101ZC – A110ZC
  • Chicken
  • Duck
  • Pork
  • Bovine
  • Sheep & goat
  • Rat
  • Horse
  • Mink
  • Fox
  • Donkey
Limit of detection 1 % (w/w); no cross-reaction observed with common species. For animal tissue, blood, foods, meat products and feed.

Foodborne pathogen kits (RUO)

7 kits · B201ZC – B207ZC
  • Staphylococcus aureus
  • Salmonella
  • Pseudomonas aeruginosa
  • Listeria monocytogenes
  • Haemolytic Streptococcus
  • Vibrio parahaemolyticus
  • Shigella
Limit of detection 10³ cfu/mL; result in 5–15 min. For foods, beverages, cosmetics, seafood and hygiene products. Method reference: GB 4789 / SN/T series.

GMO element kits (RUO)

Screening elements · on request
  • Bt
  • bar
  • hpt
  • CaMV 35S promoter
  • FMV 35S promoter
  • nos terminator
Event-specific kits (GTS 40-3-2, MON89788, MON810, Bt11, T45, Oxy-235) available on request.
17 catalogue kits — species and pathogens — plus GMO screening on requestRequest a Quote
Laboratory technician in cleanroom garments working under a laminar-flow hood

Genchek Fully Automated Isothermal Nucleic Acid Detection System

Sample-in, result-out in about 30 minutes. Pipetting, lysis, nucleic acid purification, isothermal amplification, fluorescence detection, analysis and reporting in one instrument. UV lamp and air purification built in.

Run time~30 min
Throughput1 sample
ChannelsFAM / ROX
Interface7-inch touchscreen
Weight16 kg
Dimensions260 × 325 × 420 mm
Representative laboratory image; product photo on requestRequest a Quote
Solutions

Three food-safety workflows, one 15-minute run

Isothermal RAA reagents recognise and amplify the target gene fragment; the Genchek analyzer monitors amplification in real time and the run completes in 15 minutes.

Cut of raw beef with herbs on a wooden board

Meat Species Authentication

As price gaps between meats widen, species substitution and adulteration are increasingly common. RAA-based species kits detect species-specific genes, identifying meat origin at the molecular level with no cross-reaction between species.

TargetsChicken, pork, duck, beef, sheep/goat, rat, horse, mink, fox, donkey
Sensitivity1 % adulterated meat
SamplesAnimal tissue, blood, foods, processed meat, feed
UsersFood and customs regulators, livestock authorities, agricultural institutes, food and feed manufacturers, third-party laboratories
Animal-species kits (RUO)
Gloved hand holding an agar plate with bacterial colonies

Foodborne Pathogen Detection

In China, pathogenic bacteria account for an estimated 40–50 % of reported foodborne-illness cases each year. RAA pathogen kits combine multi-channel detection, short run times and high sensitivity — raising throughput and moving food-safety warnings earlier.

TargetsS. aureus, Salmonella, P. aeruginosa, L. monocytogenes, haemolytic Streptococcus, V. parahaemolyticus, Shigella, E. coli O157:H7, V. cholerae
Sensitivity10³ cfu/mL
SamplesFoods, beverages, cosmetics, seafood
UsersFood and customs regulators, public-health laboratories, food and cosmetics manufacturers, third-party laboratories
Pathogen kits (RUO)
3D rendering of a DNA double helix in purple and teal

GMO Screening

GM crops carry genes introduced from other organisms to alter traits, nutrition or quality. Regulators worldwide require labelling and control. RAA-based GMO kits specifically detect exogenous elements and events in crops, identifying GM material at the molecular level.

ElementsBt, bar, hpt, CaMV 35S promoter, FMV 35S promoter, nos terminator
EventsSoybean GTS 40-3-2, MON89788; maize MON810, Bt11; canola T45, Oxy-235
SamplesCrops, seeds, agricultural products
UsersFood and customs regulators, agricultural institutes, food and feed manufacturers, third-party laboratories
GMO element kits (RUO)
Services

Support from first run to custom assay

Our R&D team answers every product question with patient explanation and demonstration, and provides long-term technical documentation and support.

Scientist pipetting into a petri dish beside laboratory glassware

Technical Services

Expert technical support from the team that develops the reagents — consultation, documentation, training and demonstration for every product.

  • Technical consultation and instructions for use
  • Operator training and product demonstration
  • Technical data and method documentation
Technician pipetting in a blue-lit laboratory

After-Sales Service

Good after-sales service earns customer trust. Our engineers respond promptly and resolve issues remotely — by email, online or by phone — with long-term documentation and support.

  • Remote troubleshooting for Genchek instruments
  • Service hotline +86 571 8861 9668
  • Mon–Fri 9:00–18:00 (UTC+8)
Deep blue DNA helix with molecular structure annotations

Custom Assay Development

RAA enables portable, rapid nucleic acid testing across foodborne pathogen detection, meat adulteration, GMO screening and more. We develop custom RAA assays to meet specific customer needs — contact us to discuss your target.

  • Custom targets: pathogens, species, GMO elements
  • OEM reagent supply on RAA core enzymes
  • Co-development under strategic agreements

Product operation guide — animal-species assay example

RAA is simple to run and needs no specialist. The same two-step workflow — extract, then amplify and read — applies across the catalogue.

Step 1
Weigh 50 mg samplePlace into the nucleic acid extraction solution.
Step 2
Incubate 70 °C, 10 minHeat-lysis extraction yields RAA-ready nucleic acid.
Step 3
Add buffers and sample45.5 μL Buffer A, 2.5 μL Buffer B and 2 μL sample nucleic acid into the lyophilised reagent tube.
Step 4
Read in 5–15 minPlace the strip in the Genchek analyzer; the result is read from the real-time curve.
About ZC Bioscience

An R&D-driven RAA company in Hangzhou

Hangzhou ZC Bio-Sci & Tech Co., Ltd. ("ZC Bioscience") is an R&D-driven biotechnology company based in Hangzhou, China. We develop and apply Recombinase-Aided Amplification (RAA), an isothermal nucleic acid amplification technology, and supply RAA-based reagents, extraction kits and portable fluorescence instruments for rapid molecular testing.

Since September 2016 the company has accelerated RAA product development for rapid food-safety testing, launching in turn the animal-species, foodborne pathogen and GMO ingredient molecular detection series. Further applications include environmental monitoring, aquaculture pathogen detection and animal disease detection. ZC Bioscience has signed strategic cooperation agreements with government institutions and research organisations to co-develop RAA-based detection kits.

500 m²Laboratory space, plus 400 m² of offices
RMB 12M+Installed equipment value
90 %R&D team holding master's degrees or higher
5050Hangzhou Binjiang District "5050 Talent Program" enterprise
Molecular laboratory with benches, instruments and a researcher at a workstation
Molecular laboratory
Researcher operating equipment at a laboratory bench
Sample preparation
Researcher working inside a biosafety cabinet
Biosafety cabinet
Cleanroom corridor with observation window
Cleanroom corridor
Cleanroom doors and windows along a corridor
Controlled areas
News

Latest from ZC Bioscience

Conference appearances and technical explainers on RAA.

Laboratory glassware with blue liquid on white

ZC Bioscience at the 10th China International Food Safety & Quality Conference (CBIFS 2017)

The annual CBIFS food-safety technology forum was held on 7–8 April 2017 at the Hangzhou International Expo Center. ZC Bioscience, a newcomer to food-safety testing, made its debut at the conference.

Read more
Conference hall audience seated at long tables

Forum | 7th Food Microbiology Detection & Control Technology Exchange (19–22 July)

Foodborne disease caused by pathogenic microorganisms is the number-one food-safety issue. Controlling contamination and strengthening laboratory capability have become focal concerns for regulators and food companies alike.

Read more
Visitors at an exhibition stand viewing reagent kits

Highlights | RAA Technology Debuts at the Food Microbiology Detection & Control Exchange

On 20–22 July 2017 the 7th Food Microbiology Detection and Control Technology Exchange was held in Shanghai, with 17 themed sessions, over 30 exhibitors and 300+ laboratory professionals attending.

Read more
Deep blue DNA helix illustration

Explainer | Recombinase-Aided Rapid Nucleic Acid Amplification

RAA replaces the thermal-cycling denaturation of conventional PCR with a recombinase, single-stranded DNA-binding protein and DNA polymerase, achieving rapid nucleic acid amplification at a constant 37 °C.

Read more
Contact

Request a quote or technical information

Tell us the products, quantities and destination country. We reply with a quotation, instructions for use and shipping conditions.

Hangzhou ZC Bio-Sci&Tech Co. LtdTianhe Hi-Tech Park, Bldg 5, 688 Bin'an Rd, Binjiang District, Hangzhou 310052, China
Emailzcsw2017@163.com
Telephone+86 571 8861 9668 · Mon–Fri 9:00–18:00 (UTC+8)

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Reagents are for research use only. Quotations are prepared per product and quantity.
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